Calcusyn Software Manual
CFZ had marked anti-proliferative activity in A549, H1993, H520, H460, and H1299 non-small cell lung cancer (NSCLC) cell lines, with IC 50 values after 96 hour exposure from.. These cells represent different pathological subtypes (squamous, adenocarcinoma, carcinoma) with SCLC cells derived from both metastatic lesions (SHP77) and a primary tumor (DMS114).. Alpha-tubulin antibodies were purchased from Calbiochem (La Jolla, CA) The secondary antibodies, HRP-conjugated goat anti-rabbit and HRP-conjugated goat anti-mouse, were purchased from Jackson ImmunoResearch (West Grove, PA).. A variety of molecular characteristics are also represented including wild-type p53 (H549, H460), reduced or deleted p53 (H520, H1299), wild-type KRAS (H1299), mutated KRAS (A549, H460), wild-type EGFR (A549), mutated EGFR (H1993, H460), and amplified c-met (H1993).. Reagents and antibodies CFZ, provided by Onyx Pharmaceuticals, Inc , an Amgen subsidiary (South San Francisco, CA), was dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Click
Cultures were exposed to various concentrations of CFZ or CDDP for the specified treatment intervals.. SHP77 cells were maintained in a similar manner except that heat-inactivated FBS was used. Click
3 mM CDDP in saline (Teva Pharmaceuticals, Israel) was stored at 20C Antibodies against poly ADP ribose polymerase (PARP), cleaved caspase-3, p-glycoprotein (Pgp; MDR1), and B-cell lymphoma 2 (Bcl-2) were purchased from Cell Signaling Technology (Beverly, MA).. All cells were cultured in RPMI 1640 (Cellgro) with 10% fetal bovine serum (FBS), 1 mM sodium pyruvate, and 10 mM HEPES.. Autosomal short tandem repeat typing was conducted across the 13 core STRs in CODIS and referenced against allelic peaks in cell lines of previously confirmed genotype.. Louis, MO) at a stock concentration of 10 mM and stored at 20C A stock concentration of 3.. All cells were grown in 5% CO 2 at 37C in a humidified tissue culture incubator. https://hub.docker.com/r/ellirespples/dragon-for-mac-5-reviews
All cell lines were verified as authentic Proliferation assay Cells were seeded in 96-well plates and incubated overnight. 773a7aa168 4
Proliferation of suspension cells was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay.. Proliferation of adherent cells was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.. Antibodies against microtubule-associated protein-1 light chain-3B (LC3B) were obtained from Sigma-Aldrich. HERE
